HBMEC細(xì)胞|美國(guó)Sciencell|人腦微血管內(nèi)皮細(xì)胞 全國(guó)統(tǒng)一價(jià)格，全國(guó)各地均可發(fā)貨，遠(yuǎn)到香港、澳門、福建、新疆、內(nèi)蒙古等地。

產(chǎn)品名稱：HBMEC細(xì)胞

中文名稱：人腦微血管內(nèi)皮細(xì)胞

來(lái)源：美國(guó)Sciencell

生長(zhǎng)狀態(tài)：貼壁生長(zhǎng)|懸浮生長(zhǎng)|半貼壁半懸浮生長(zhǎng)

細(xì)胞形態(tài)：上皮樣|成纖維樣|圓形|不規(guī)則

培養(yǎng)條件：DMEM|1640|MEM|IMDM+10% FBS

細(xì)胞規(guī)格：5 x 10^5 cells/vial

特征特性：

人腦微血管內(nèi)皮細(xì)胞是血腦屏障的主要組成成分，能夠限制可溶性物質(zhì)和細(xì)胞等從血液進(jìn)入大腦。大腦微血
管內(nèi)皮細(xì)胞與外周內(nèi)皮細(xì)胞相比具有一些相同特性。如：1）腦微血管內(nèi)皮細(xì)胞存在許多細(xì)胞間緊密連接，
產(chǎn)生很高的跨內(nèi)皮阻抗，延遲細(xì)胞旁的通量；2）腦微血管內(nèi)皮細(xì)胞缺乏內(nèi)皮細(xì)胞的窗孔結(jié)構(gòu)，其液相物質(zhì)
胞飲水平較低；3）腦微血管內(nèi)皮細(xì)胞具有不對(duì)稱定位酶和載體介導(dǎo)轉(zhuǎn)運(yùn)系統(tǒng)，從而產(chǎn)生 “兩極分化”的表現(xiàn)型。
與外周內(nèi)皮細(xì)胞相同，大腦微血管內(nèi)皮細(xì)胞表面表達(dá)細(xì)胞粘附分子，調(diào)控白細(xì)胞進(jìn)入大腦。由于微血管內(nèi)皮細(xì)
胞的器官特異性，內(nèi)皮細(xì)胞通常取源于疾病研究的相關(guān)組織。

HBMEC細(xì)胞提取于人腦組織，提取純化之后立即凍存。每管含有細(xì)胞數(shù) >5×105 cells/ml，
此細(xì)胞通過(guò)vWF/Factor VIII 和CD31 (P-CAM)免疫熒光染色驗(yàn)證，經(jīng)測(cè)試不含有HIV-1、HBV、HCV、支原體、
細(xì)菌、酵母和真菌。

庫(kù)存狀態(tài)：現(xiàn)貨

運(yùn)輸方式：運(yùn)輸方式分為新鮮運(yùn)輸和凍存管運(yùn)輸兩種運(yùn)輸方式

貨期：新鮮運(yùn)輸需要1-2周，凍存管運(yùn)輸?shù)男枰?-3天

慧穎細(xì)胞庫(kù)提供的所有細(xì)胞僅供科研使用

原代細(xì)胞的培養(yǎng)是指直接從機(jī)體取下細(xì)胞、組織和器官后立即進(jìn)行培 養(yǎng)；因此，較為嚴(yán)格地說(shuō)是指成功傳代之前的培養(yǎng)，此時(shí)的細(xì)胞保持原有細(xì)胞的基本性質(zhì)，如果是正常細(xì)胞，仍然保留二倍體數(shù)。不同的原代細(xì)胞傳代次數(shù)不同，具體需咨詢客服人員或者自行查閱 相關(guān)資料。

細(xì)胞株是通過(guò)選擇法或克隆形成法從原代培養(yǎng)物或細(xì)胞系中獲得的具有特殊性質(zhì)或標(biāo)志的培 養(yǎng)細(xì)胞。從培養(yǎng)代數(shù)來(lái)講，理論上可培養(yǎng)到40-50代。

客戶應(yīng)具有一定細(xì)胞培養(yǎng)知識(shí)與經(jīng)驗(yàn)，并具備基本培養(yǎng)條件（細(xì)胞培養(yǎng)實(shí)驗(yàn)室，無(wú)菌操作臺(tái)、CO2培養(yǎng)箱，可拍照倒置顯微鏡等），并按說(shuō)明書要求準(zhǔn)備好相應(yīng)的無(wú)菌的培養(yǎng)基、血清、雙抗、細(xì)胞培養(yǎng)瓶、培養(yǎng)板等。如果因客戶缺少基本培養(yǎng)經(jīng)驗(yàn)和培養(yǎng)條件而導(dǎo)致細(xì)胞死亡或污染，不在我司售后責(zé)任之內(nèi)。

相關(guān)HBMEC細(xì)胞|美國(guó)Sciencell|人腦微血管內(nèi)皮細(xì)胞熱賣產(chǎn)品：

骨、關(guān)節(jié)、軟組織和皮膚系統(tǒng)Bones, joints,soft tissue and skin system

BJ細(xì)胞，人原代成纖維細(xì)胞         5 × 105         1-2weeks

HSF細(xì)胞，      人皮膚成纖維細(xì)胞         5 × 105         1-2weeks

FLS細(xì)胞，       成纖維樣滑膜細(xì)胞         5 × 105         1-2weeks

A-431細(xì)胞，  人皮膚鱗癌細(xì)胞     5 × 105         1-2weeks

SK-MEL-1 細(xì)胞，人皮膚黑色素瘤細(xì)胞         5 × 105         1-2weeks

A875細(xì)胞，   人黑色素瘤細(xì)胞     5 × 105         1-2weeks

A-375細(xì)胞，  人惡性黑色素瘤細(xì)胞     5 × 105         1-2weeks

HaCaT細(xì)胞，          人永生化表皮細(xì)胞         5 × 105         1-2weeks

hFOB1.19          細(xì)胞，人永生化成骨細(xì)胞     5 × 105         1-2weeks

hFOB 1.19細(xì)胞，   SV40轉(zhuǎn)染人成骨細(xì)胞    5 × 105         1-2weeks

HT-1080細(xì)胞，人纖維肉瘤細(xì)胞  5 × 105         1-2weeks

MG-63細(xì)胞，人成骨肉瘤細(xì)胞     5 × 105         1-2weeks

Saos-2細(xì)胞， 人成骨肉瘤細(xì)胞     5 × 105         1-2weeks

TE671 subline No.2 人橫紋肌肉瘤細(xì)胞         5 × 105         1-2weeks

U266細(xì)胞，   人骨髓瘤細(xì)胞         5 × 105         1-2weeks

HOS細(xì)胞，  人骨肉瘤細(xì)胞          5 × 105         1-2weeks

U-2 OS細(xì)胞，人骨肉瘤細(xì)胞          5 × 105         1-2weeks

h-BMSC細(xì)胞，人骨髓間質(zhì)細(xì)胞   5 × 105         1-2weeks

呼吸系統(tǒng)respiratory system

16HBE      人支氣管上皮樣細(xì)胞     5 × 105         1-2weeks

95-D          人高轉(zhuǎn)移肺癌細(xì)胞         5 × 105         1-2weeks

H322         肺癌細(xì)胞         5 × 105         1-2weeks

A549         人肺癌細(xì)胞     5 × 105         1-2weeks

H1299(中科：NCI-H1299）   人肺癌細(xì)胞     5 × 105         1-2weeks

PC9  人肺癌細(xì)胞     5 × 105         1-2weeks

PC14         人肺癌細(xì)胞     5 × 105         1-2weeks

SPCA-1     人肺腺癌細(xì)胞         5 × 105         1-2weeks

Calu-3       人肺腺癌細(xì)胞         5 × 105         1-2weeks

LTEP-a-2   人肺腺癌細(xì)胞         5 × 105         1-2weeks

MRC-5      人胚肺成纖維細(xì)胞         5 × 105         1-2weeks

WI-38       人胚胎肺成纖維細(xì)胞     5 × 105         1-2weeks

A549（成瘤）         人非小細(xì)胞肺癌細(xì)胞     5 × 105         1-2weeks

NCI-H358          人非小細(xì)胞肺癌細(xì)胞     5 × 105         1-2weeks

NCI-H1650       人非小細(xì)胞肺癌細(xì)胞     5 × 105         1-2weeks

NCI-H1975       人非小細(xì)胞肺腺癌細(xì)胞         5 × 105         1-2weeks

NCI-N838          人非小細(xì)胞肺癌細(xì)胞     5 × 105         1-2weeks

SK-MES-1 人肺鱗癌細(xì)胞         5 × 105         1-2weeks

NCI-H226          人肺鱗癌細(xì)胞         5 × 105         1-2weeks

NCI-H520          人肺鱗癌細(xì)胞         5 × 105         1-2weeks

NCI-H460          人大細(xì)胞肺癌細(xì)胞         5 × 105         1-2weeks

NCI-H661          人大細(xì)胞肺癌細(xì)胞         5 × 105         1-2weeks

泌尿系統(tǒng)urinary system

293  人胚腎細(xì)胞     5 × 105         1-2weeks

293A         人胚腎細(xì)胞     5 × 105         1-2weeks

293F          人胚腎細(xì)胞     5 × 105         1-2weeks

HEK293    人胚腎細(xì)胞     5 × 105         1-2weeks

293XL-hTLR7    人胚腎細(xì)胞     5 × 105         1-2weeks

293XL-hTLR9    人胚腎細(xì)胞     5 × 105         1-2weeks

293-mTLR5     人胚腎細(xì)胞     5 × 105         1-2weeks

293 xhTLR7      人胚腎細(xì)胞     5 × 105         1-2weeks

293 xhTLR9      人胚腎細(xì)胞     5 × 105         1-2weeks

293H         人胚腎細(xì)胞     5 × 105         1-2weeks

293FT       人胚腎細(xì)胞-用于慢病毒包裝        5 × 105         1-2weeks

293TN      人胚腎細(xì)胞--用于慢病毒包裝       5 × 105         1-2weeks

293T/17   人胚腎細(xì)胞-用于慢病毒包裝        5 × 105         1-2weeks

293LVT     人胚腎細(xì)胞--用于慢病毒包裝       5 × 105         1-2weeks

293T         人胚腎細(xì)胞（SV40T基因修飾） 5 × 105         1-2weeks

LNCaP clone FGC     人前列腺癌細(xì)胞     5 × 105         1-2weeks

DU 145     人前列腺癌細(xì)胞     5 × 105         1-2weeks

786-O       人腎透明細(xì)胞癌細(xì)胞     5 × 105         1-2weeks

Caki-1       人腎透明細(xì)胞癌皮膚轉(zhuǎn)移細(xì)胞     5 × 105         1-2weeks

G401         人腎癌Wilms細(xì)胞          5 × 105         1-2weeks

HK-2          人腎皮質(zhì)近曲小管上皮細(xì)胞         5 × 105         1-2weeks

ADPC        人雄激素依靠性前列腺癌細(xì)胞     5 × 105         1-2weeks

RWPE-1    人正常前列腺上皮細(xì)胞         5 × 105         1-2weeks

T24  人膀胱癌細(xì)胞         5 × 105         1-2weeks

EJ     人膀胱癌細(xì)胞         5 × 105         1-2weeks

5637         人膀胱癌細(xì)胞         5 × 105         1-2weeks

ScaBER     人膀胱鱗癌細(xì)胞     5 × 105         1-2weeks

內(nèi)分泌系統(tǒng)endocrine system

BT-474     人乳腺導(dǎo)管癌細(xì)胞         5 × 105         1-2weeks

435（中科：MDA-MB-435S ）     人乳腺導(dǎo)管癌         5 × 105         1-2weeks

HS-578T   人乳腺癌細(xì)胞         5 × 105         1-2weeks

MCF-7      人乳腺癌細(xì)胞         5 × 105         1-2weeks

MDA-MB-231  人乳腺癌細(xì)胞         5 × 105         1-2weeks

T47D         人乳腺癌細(xì)胞         5 × 105         1-2weeks

LLC   人乳腺癌細(xì)胞         5 × 105         1-2weeks

MCF 7       人乳腺癌細(xì)胞         5 × 105         1-2weeks

MDA-MB-231  人乳腺癌細(xì)胞         5 × 105         1-2weeks

SKBR3       人乳腺腺癌細(xì)胞     5 × 105         1-2weeks

MCF 10A  人正常乳腺上皮細(xì)胞     5 × 105         1-2weeks

SW579      人甲狀腺鱗癌細(xì)胞         5 × 105         1-2weeks

TT     人髓狀甲狀腺腫瘤細(xì)胞         5 × 105         1-2weeks

腦和神經(jīng)系統(tǒng)The brain and nervous system

A172         人膠質(zhì)母細(xì)胞瘤細(xì)胞     5 × 105         1-2weeks

BE(2)-M17        人神經(jīng)母細(xì)胞瘤細(xì)胞     5 × 105         1-2weeks

IMR-32     人神經(jīng)母細(xì)胞瘤細(xì)胞     5 × 105         1-2weeks

SH-SY5Y   人神經(jīng)母細(xì)胞瘤細(xì)胞     5 × 105         1-2weeks

SK-N-SH    人神經(jīng)母細(xì)胞瘤細(xì)胞     5 × 105         1-2weeks

SHG-44     人膠質(zhì)瘤細(xì)胞         5 × 105         1-2weeks

HS 683      人腦膠質(zhì)瘤細(xì)胞     5 × 105         1-2weeks

U-373MG          人腦神經(jīng)膠質(zhì)瘤細(xì)胞     5 × 105         1-2weeks

U251         人神經(jīng)膠質(zhì)細(xì)胞瘤細(xì)胞         5 × 105         1-2weeks

U-87 MG  人腦星形膠質(zhì)母細(xì)胞瘤細(xì)胞         5 × 105         1-2weeks

生殖系統(tǒng)reproductive system

C-33A       人宮頸癌細(xì)胞         5 × 105         1-2weeks

Ca Ski      人宮頸癌細(xì)胞         5 × 105         1-2weeks

Hela229   人宮頸癌細(xì)胞         5 × 105         1-2weeks

HeLa         人宮頸癌細(xì)胞         5 × 105         1-2weeks

         人胚胎成纖維細(xì)胞         5 × 105         1-2weeks

HEC-1-B   人子宮內(nèi)膜腺癌細(xì)胞     5 × 105         1-2weeks

Ishikawa  人子宮內(nèi)膜癌細(xì)胞         5 × 105         1-2weeks

SKOV3       人卵巢癌細(xì)胞         5 × 105         1-2weeks

HO-8910  人卵巢癌細(xì)胞         5 × 105         1-2weeks

SK-OV-3    人卵巢腺癌細(xì)胞     5 × 105         1-2weeks

OVCAR-3 人卵巢腺癌細(xì)胞     5 × 105         1-2weeks

JEG3         人絨毛膜癌細(xì)胞     5 × 105         1-2weeks

Ms751      人子宮頸表皮癌細(xì)胞     5 × 105         1-2weeks

Siha  人子宮頸鱗癌細(xì)胞         5 × 105         1-2weeks

KLE   人子宮內(nèi)膜腺癌細(xì)胞     5 × 105         1-2weeks

消化系統(tǒng)digestive system

PANC-1細(xì)胞，人胰腺癌細(xì)胞        5 × 105         1-2weeks

SW1990細(xì)胞，       人胰腺癌細(xì)胞         5 × 105         1-2weeks

BxPC-3細(xì)胞，人胰腺腺癌細(xì)胞    5 × 105         1-2weeks

chang liver細(xì)胞，  人張氏肝細(xì)胞         5 × 105         1-2weeks

HIC-2細(xì)胞，  人小腸癌細(xì)胞         5 × 105         1-2weeks

HCT-8細(xì)胞，人回盲腸癌細(xì)胞      5 × 105         1-2weeks

SW620[SW-620] 細(xì)胞，人結(jié)腸癌細(xì)胞          5 × 105         1-2weeks

HCT-116細(xì)胞，人結(jié)腸癌細(xì)胞      5 × 105         1-2weeks

COLO 205         細(xì)胞，人結(jié)腸癌細(xì)胞     5 × 105         1-2weeks

RKO細(xì)胞，人結(jié)腸腺癌細(xì)胞 5 × 105         1-2weeks

LoVo細(xì)胞，人結(jié)直腸腺癌細(xì)胞    5 × 105         1-2weeks

HT-29細(xì)胞，  人結(jié)直腸腺癌細(xì)胞         5 × 105         1-2weeks

Caco-2細(xì)胞，人結(jié)直腸腺癌細(xì)胞         5 × 105         1-2weeks

SW480細(xì)胞，人結(jié)直腸腺癌細(xì)胞         5 × 105         1-2weeks

SW620細(xì)胞，人結(jié)直腸腺癌細(xì)胞         5 × 105         1-2weeks

SW837細(xì)胞，人結(jié)直腸腺癌上皮細(xì)胞         5 × 105         1-2weeks

DLD-1細(xì)胞， 人結(jié)直腸腺癌上皮細(xì)胞         5 × 105         1-2weeks

KYSE30細(xì)胞，人食管鱗癌細(xì)胞    5 × 105         1-2weeks

KYSE-150 細(xì)胞，人食管鱗癌細(xì)胞         5 × 105         1-2weeks

KYSE-450細(xì)胞，人食管鱗癌細(xì)胞          5 × 105         1-2weeks

TE-1 細(xì)胞，人食管癌細(xì)胞     5 × 105         1-2weeks

ECA109細(xì)胞，人食管癌細(xì)胞        5 × 105         1-2weeks

EC9706細(xì)胞，人食管癌細(xì)胞        5 × 105         1-2weeks

MH97H細(xì)胞，人高轉(zhuǎn)移潛能肝癌細(xì)胞株   5 × 105         1-2weeks

HCCLM3細(xì)胞，      人高轉(zhuǎn)移肝癌細(xì)胞         5 × 105         1-2weeks

SMMC-7721細(xì)胞，人肝癌細(xì)胞    5 × 105         1-2weeks

BEL-7404 細(xì)胞，人肝癌細(xì)胞         5 × 105         1-2weeks

Hep G2細(xì)胞，人肝癌細(xì)胞    5 × 105         1-2weeks

Hep-3B細(xì)胞，人肝癌細(xì)胞    5 × 105         1-2weeks

BEL-7402 細(xì)胞，人肝癌細(xì)胞         5 × 105         1-2weeks

BEL-7404 細(xì)胞，人肝癌細(xì)胞         5 × 105         1-2weeks

SNU-739  細(xì)胞，人肝癌細(xì)胞         5 × 105         1-2weeks

HepG2細(xì)胞，人肝癌細(xì)胞     5 × 105         1-2weeks

97L細(xì)胞，人肝癌細(xì)胞  5 × 105         1-2weeks

HuH-7細(xì)胞， 人肝癌細(xì)胞     5 × 105         1-2weeks

QGY-7703細(xì)胞，人肝癌細(xì)胞        5 × 105         1-2weeks

HOC細(xì)胞，   肝卵圓細(xì)胞     5 × 105         1-2weeks

L-02（HL-7702) 細(xì)胞， 人正常肝細(xì)胞         5 × 105         1-2weeks

QSG-7701細(xì)胞，    人正常肝細(xì)胞         5 × 105         1-2weeks

GES-1細(xì)胞，  人胃上皮細(xì)胞         5 × 105         1-2weeks

MGC-803細(xì)胞，人胃癌細(xì)胞         5 × 105         1-2weeks

MKN-45細(xì)胞，人胃癌細(xì)胞  5 × 105         1-2weeks

AGS細(xì)胞，     人胃癌細(xì)胞     5 × 105         1-2weeks

AZ-521細(xì)胞，人胃癌細(xì)胞    5 × 105         1-2weeks

Hs 746T細(xì)胞，人胃癌細(xì)胞  5 × 105         1-2weeks

NUGC-3細(xì)胞，人胃癌細(xì)胞  5 × 105         1-2weeks

SNU-638細(xì)胞，人胃癌細(xì)胞 5 × 105         1-2weeks

NCI-N87細(xì)胞，人胃癌細(xì)胞  5 × 105         1-2weeks

HGC-27細(xì)胞，人胃癌細(xì)胞（未分化）        5 × 105         1-2weeks

BGC-823  細(xì)胞，人胃腺癌細(xì)胞     5 × 105         1-2weeks

SGC-7901          細(xì)胞，人胃腺癌細(xì)胞     5 × 105         1-2weeks

GBC-SD細(xì)胞，人膽囊癌細(xì)胞        5 × 105         1-2weeks

QBC939細(xì)胞，       人膽管癌細(xì)胞         5 × 105         1-2weeks

血液和淋巴系統(tǒng)Blood and lymphatic system

6TCEM細(xì)胞，人T細(xì)胞白血病細(xì)胞    5 × 105         1-2weeks

JURKAT細(xì)胞，人T淋巴細(xì)胞白血病細(xì)胞株        5 × 105         1-2weeks

CCRF-CEM細(xì)胞，  人急性淋巴細(xì)胞白血病T淋巴細(xì)胞     5 × 105         1-2weeks

MOLT-4細(xì)胞，人急性淋巴母細(xì)胞白血病細(xì)胞   5 × 105         1-2weeks

REH 細(xì)胞，急性淋巴細(xì)胞白血病         5 × 105         1-2weeks

supb15細(xì)胞，急淋白血病細(xì)胞系         5 × 105         1-2weeks

K-562細(xì)胞，  人慢性髓原白血病細(xì)胞         5 × 105         1-2weeks

K562細(xì)胞，人慢性髓性白血病細(xì)胞    5 × 105         1-2weeks

HL-60細(xì)胞，  白血病細(xì)胞     5 × 105         1-2weeks

TF-1細(xì)胞，人血液白血病細(xì)胞     5 × 105         1-2weeks

HSPC-1細(xì)胞，人造血干細(xì)胞         5 × 105         1-2weeks

Daudi細(xì)胞，  人Burkitt淋巴瘤細(xì)胞    5 × 105         1-2weeks

NAMALWA細(xì)胞，  人Burkitt淋巴瘤細(xì)胞    5 × 105         1-2weeks

Raji細(xì)胞 人Burkitt淋巴瘤細(xì)胞    5 × 105         1-2weeks

RAMOS細(xì)胞，人B淋巴細(xì)胞瘤細(xì)胞   5 × 105         1-2weeks

HUVEC[HUV-EC-C] 細(xì)胞，    人臍靜脈內(nèi)皮細(xì)胞         5 × 105         1-2weeks

MPCs細(xì)胞，  單核巨噬細(xì)胞         5 × 105         1-2weeks

THP-1細(xì)胞，  急性單核白血病細(xì)胞     5 × 105         1-2weeks

U937細(xì)胞，   人組織細(xì)胞淋巴瘤細(xì)胞         5 × 105         1-2weeks

HCAEC細(xì)胞，人冠狀動(dòng)脈內(nèi)皮細(xì)胞    5 × 105         1-2weeks

HemSc細(xì)胞，血管瘤干細(xì)胞         5 × 105         1-2weeks

             人淋巴細(xì)胞     5 × 105         1-2weeks

循環(huán)系統(tǒng)circulatory system

HUV-EC細(xì)胞，人臍靜脈內(nèi)皮細(xì)胞       5 × 105         1-2weeks

眼、耳、鼻、喉和口腔系統(tǒng)Eye, ear, nose, throat and mouth system

CAL-27細(xì)胞，人舌鱗癌細(xì)胞         5 × 105         1-2weeks

Tca-8113細(xì)胞，     人舌鱗癌細(xì)胞         5 × 105         1-2weeks

HDPC細(xì)胞，  人牙髓細(xì)胞     5 × 105         1-2weeks

         人牙周膜細(xì)胞         5 × 105         1-2weeks

PDLC細(xì)胞，   牙周膜成纖維細(xì)胞         5 × 105         1-2weeks

CNE-2Z細(xì)胞，人鼻咽癌細(xì)胞        5 × 105         1-2weeks

          人耳周膜細(xì)胞       5 × 105         1-2weeks

SCC-9細(xì)胞，  人口腔癌細(xì)胞         5 × 105         1-2weeks

KB細(xì)胞，人口腔表皮樣癌細(xì)胞   5 × 105         1-2weeks

收到處理方式：

新鮮細(xì)胞到貨處理方式，客戶收到細(xì)胞后按以下方式操作：

取出25cm2培養(yǎng)瓶，75%酒精消毒，拆下封口膜，放入37℃，5% CO2細(xì)胞培養(yǎng)箱中靜置2-3h，以穩(wěn)定細(xì)胞狀態(tài)。

待細(xì)胞達(dá)到80%匯合時(shí)準(zhǔn)備進(jìn)行傳代培養(yǎng)。

細(xì)胞傳代，吸出25cm2培養(yǎng)瓶中的培養(yǎng)基,用PBS清洗細(xì)胞一次；添加0.25%胰蛋白酶消化液約1ml至培養(yǎng)瓶中，37℃溫浴3min左右；倒置顯微鏡下觀察，待細(xì)胞回縮變圓后吸棄消化液，再加入*培養(yǎng)液終止消化；用吸管輕輕吹打混勻，按1:2適當(dāng)?shù)谋壤M(jìn)行接種傳代（原代細(xì)胞必須以1:2方式傳代），然后補(bǔ)充新鮮的*培養(yǎng)基至5ml，放入37℃，5% CO2細(xì)胞培養(yǎng)箱中培養(yǎng)；待細(xì)胞*貼壁后，培養(yǎng)觀察。之后每隔2-3天更換新鮮的*培養(yǎng)基。

凍存管到貨后處理方式，客戶收到細(xì)胞產(chǎn)品后，請(qǐng)按照下述方法操作：

細(xì)胞凍存管公司多贈(zèng)送一管，建議客戶先復(fù)蘇1支，如果復(fù)蘇效果不理想，先與技術(shù)人員溝 通后再?gòu)?fù)蘇另1支。

細(xì)胞收到后盡快復(fù)蘇，有效期為一周。

收到凍存管后如不馬上復(fù)蘇，可以暫時(shí)放置于-80℃冰箱，不建議放入液氮罐。

復(fù)蘇所用血清必須是胎牛血清。

復(fù)蘇請(qǐng)按照細(xì)胞復(fù)蘇 SOP 方式操作（細(xì)胞復(fù)蘇 SOP 另行發(fā)送）。

熱賣：DC2.4細(xì)胞，SW48細(xì)胞，RS4:11細(xì)胞，KGN細(xì)胞，A2780細(xì)胞，A2780/DDP細(xì)胞，HL-1細(xì)胞，COV434細(xì)胞等

更多人卵巢癌細(xì)胞系、人肺癌細(xì)胞系、人結(jié)腸癌細(xì)胞系、人肝癌細(xì)胞系、人膀胱癌細(xì)胞系 耐藥株 盡在慧穎細(xì)胞庫(kù)

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：王

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